Sorry, should've clarified. I was referring to say for example having a booster or compressor ON all the time. that's what I meant.
However, boss pedals as I understand have an "always ON buffer", so they buffer the signal regardless of whether the pedal state is ON or OFF. Of course, so long as they have power. But I recall the buffer in Boss pedals didn't really rejuvenate the signal like a standalone buffer does. Perhaps everyone's experience is different and of course everyone's ears are different as well, but to my ears at the time, they took some high end out of my tone, at least it was enough to be noticeable to me... and I remember doing the test with a 10 foot Mogami 2524 cable into a boss pedal and then a 20 foot Mogami 2524 into the front of the amp.
I remember trying an SD-1, DS-1, TU2, NS2. sadly, they all had similar results with the high end.
Cheers