Most commercially available guitar amps are set up so that the tubes that run in pairs are 1 & 4, and 2 & 3.
I have never seen a power section of a four power tube guitar amp done any other way.
This is not to say that it can't be done as your is, but i have never heard of it being done that way.
In "most" amps, if you pull two power tubes, you must reduce the ohms by one half as well when plugging in.
That is to say, in a four power tube amp which uses a 16 ohm load to the cab, if you pull a pair of tubes, you now need to plug in at 8 ohms.
Some power transformers can handle this tube pulling without any need to change the ohms, but consult with the manufacturer prior to just doing it.