It depends on the guitar. I have an R8 that needs an F-spaced pickup. My other LPs do not.
In my experience, the spacing matters. If the pole spacing is too narrow, my up-strokes in the low E string sound weak and splatty (down stroke sounds fine).   It's worse the lower the string tension gets.  I've confirmed that it's the pole spacing by jamming something into the pickup ring to force the pole under the low E string, and the problem goes away.
So for me, I've got to have a properly spaced bridge pickup.